{"id":1064,"date":"2021-03-31T11:54:11","date_gmt":"2021-03-31T11:54:11","guid":{"rendered":"https:\/\/www.ivb.ro\/?page_id=1064"},"modified":"2024-03-11T10:09:49","modified_gmt":"2024-03-11T10:09:49","slug":"echipamentele-laboratorului-de-biochimie-proteomica","status":"publish","type":"page","link":"https:\/\/www.ivb.ro\/en\/echipamentele-laboratorului-de-biochimie-proteomica","title":{"rendered":"The equipment of the laboratory of Biochemistry and Proteomics"},"content":{"rendered":"
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The Equipment Of The Laboratory<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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4D - Nucleofactor<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u00a0<\/p>

Unit of nucleofection for the tanks and the plates of the 16 wells, with programs that are pre-defined for the cell lines adherent and suspension, and primary cultures, the ability to optimize, and export it easy for the data.
It presents flexibility for the different substrates, the end-to-end to the complex, the NFA, and the peptide.
Applications: supraexpresie or silen\u021biere gene expression of the protein, the generation of a clone of the stable. The supplies are dedicated to him.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Hood with laminar flow - Holten MaxiSafe 2010<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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class IIA, which has the following characteristics:<\/span><\/p>

\u2013 2 HEPA filters in the upper part of a main filter and a filter to the exhaust.<\/span><\/p>

\u2013 UV lamp built-in program and of the standard of disinfection, <\/span><\/p>

\u2013 anti-UV, and the panel is activated, the electric <\/span><\/p>

the wall of glass, with a thickness of 6 mm, which is operated by means of a digital board with a microprocessor-based <\/span><\/p>

\u00a0<\/p>

\u2013 LCD display, an indicator of the status of the flow laminar, with a built-in alarm.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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An Incubator with an atmosphere of CO2 - ShelLab<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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With a dual lead frame housing to the top of the water, it is ideal for cell culture. The design features a uniform temperature, which prevents the sudden change, and the shock of the heat cell, as well as an atmosphere of constant CO -2<\/sub>easy-to-maintain and control.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Inverted microscope - Eclipse TS-100 for Nikon <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Phase-contrast and light from the fluorescent useful for the study of cell cultures and for the of the if. The source of the light provided by the LED lights green, with a low consumption and a long operating life, up to 60,000 hours.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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The Platform Of The Luminex 200 <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The device allows determination of the type of plywood<\/em><\/strong> \u2013 up to 100\/500 analytes in a single sample. The technology can also be used in the determination of the profile of the expression of the protein, with the expansion into the genomic and the profiling of gene expression. The system includes a device for reading the Luminex x-y\u2122, the delivery of working fluid to the Luminex SD\u2122 software (xPonent 3.1 in) and a computer.<\/span><\/p>

The technology xMAP (Multi-Analyte Profiling) introduces an innovative solution ap-marked, fluorescent, the type of bonding to the molecules of interest, using a patented process of incorporating a unique mix out of a 2-or 3-fluorocromi. Depending on the extent of incorporation of these fluorocromi, with a range of reading, in the red and near-infrared can be obtained in 100 of the population that is distinct from the microspheres of polystyrene\/500 of the population, in the case of microspheres of polystyrene magnetic properties.<\/span><\/p>

Multiplexing xMAP array is a technology, proteomics, advanced, allowing for the identification and simultaneous quantification of biomarker panels for x-plex in a very small volume of biological sample (serum, plasma, supernatant cell phone, used cell\/tissue, urine, cerebrospinal fluid, etc.) in a relatively short period of time, with a high level of accuracy, while offering the advantage of a wide range of impressive applications in the sphere of scientific research and in the clinical setting.<\/span><\/p>

Applications: <\/u><\/strong><\/span><\/p>

  • the analysis of the sets of x-plex cytokine pro - \/anti-inflammatory properties for the assessment of the effects of anti-inflammatory, anti-pain of the bioprodu\u0219ilor,<\/span><\/li>
  • the analysis of the molecules of the signal in order to evaluate the modulation of intracellular signaling by the action of the bioprodu\u0219ilor,<\/span><\/li>
  • the investigation of the foundations of the molecular of the bio-security and the effectiveness of the bioprodu\u0219ilor,<\/span><\/li>
  • the creation of a comprehensive system for the investigation on the implementation of the instruments in proteomics for the assessment of the profile of the immunotoxicity for bioproducts.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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    Microplate Multimode Detector, Zenyth 3100 (Platform, Anthos Zenyth) <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    The equipment supports almost all types of applications in molecular biology, biochemistry, environmental tests, and nutrition, toxicology, and the screening of drugs.<\/span><\/p>

    Applications and technologies for the detection, reading the absorbance and the fluorescence read-polarized light, TiRF, FRET, the absorbance of UV\/VIS. It will be used for the quantification of proteins and nucleic acids, the study of the interaction of the protein-protein (receptor-ligand), test enzimologice, quizzes, a reporter, the determination of proteins phosphorylated, activity of the enzyme.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    System-vertical-electrophoresis-and-transfer Miniprotean II, BioRad (Western blot) <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    The system is used at its maximum capacity to allow the separation of a maximum of 56 of the evidence at the same time. The gels obtained, they can be stained and analyzed, and may be subject to a further transfer on the membrane, and thus for the identification of the specific protein in the imunodetec\u021bie (western blot). The identification of a western blot is done by reading the result of using the scanner for the detection of chemiluminiscen\u021bei c-Code.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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    Equipment Typhoon FLA 9000 <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    Laser scanner, which is useful for imaging applications in biomolecular, including the measurement of isotope ratios, fluorocromi or the signal is out of chemiluminescence with high sensitivity.<\/span><\/p>

    Applications: the 2-D DIGE, fluorescent multiplex (in the visible and NIR, near infrared), and western blot chemiluminescence, as well as the staining of the gel (Coomassie\u2122 blue and soak argentic\u0103).<\/span><\/p>

    Features: - capture signal radioizotoipic, fluorescent, or chemiluminescent, high-resolution, and quantization can be optimized by the addition of a metal detector, filtere, and lasers, and high-throughput detection for the simultaneous of up to 16 bloturi or gels, 10 x 8 inches, 2-D DIGE image.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    The C-DiGit Blot Scanner, LICOR <\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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    Equipment, technique, multiscan, multi-exposure, which gives you the opportunity to pick up the signals of chemiluminescence by linear scanning.<\/span><\/p>

    The short distance between the photon source and the detector, making it possible to detect for quick and precise signal of the chemiluminescence. To do this, contribute, and the linear scan, which captures more light signals, and makes it unnecessary to superr\u0103cirea on which it is based, the digital cameras of the new generation.<\/span><\/p>

    The device is small and portable, does not require a darkroom or film, radiographic, acquiring a picture directly from the blotare.<\/span><\/p>

    The resolution of the device it is out of 196 \u00b5m \u00d7 196 mm and has a two-stroke engine of the procurement: the standard, a 6-minute, high -, and 12 minutes. The resulting image can be adjusted at any time, from exposure to the intermediary, in this time period. This scanner is accompanied by a dedicated laptop with the software, your own, easy-to-use.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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    Echipamentele Laboratorului 4D – Nucleofactor \u00a0 Unitatate de nucleofec\u021bie pentru cuve \u0219i pl\u0103ci de 16 godeuri, cu programe predefinite pentru linii celulare aderente \u0219i \u00een suspensie \u0219i culturi primare, posibilitate de optimizare \u0219i export facil de date.Prezint\u0103 flexibilitate pentru diverse substraturi, de la plasmide la complexe RNP \u0219i peptide. Aplica\u021bii: supraexpresie sau silen\u021biere genic\u0103, expresie… <\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-1064","page","type-page","status-publish","hentry"],"yoast_head":"\nEchipamentele laboratorului de Biochimie-Proteomica - National Institute of Pathology Victor Babe\u015f - Bucharest<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.ivb.ro\/en\/echipamentele-laboratorului-de-biochimie-proteomica\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Echipamentele laboratorului de Biochimie-Proteomica - National Institute of Pathology Victor Babe\u015f - Bucharest\" \/>\n<meta property=\"og:description\" content=\"Echipamentele Laboratorului 4D – Nucleofactor \u00a0 Unitatate de nucleofec\u021bie pentru cuve \u0219i pl\u0103ci de 16 godeuri, cu programe predefinite pentru linii celulare aderente \u0219i \u00een suspensie \u0219i culturi primare, posibilitate de optimizare \u0219i export facil de date.Prezint\u0103 flexibilitate pentru diverse substraturi, de la plasmide la complexe RNP \u0219i peptide. 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