{"id":12517,"date":"2025-07-25T06:24:41","date_gmt":"2025-07-25T06:24:41","guid":{"rendered":"https:\/\/www.ivb.ro\/?page_id=12517"},"modified":"2025-07-25T06:48:01","modified_gmt":"2025-07-25T06:48:01","slug":"pn-iii-p4-pce-2021-1680","status":"publish","type":"page","link":"https:\/\/www.ivb.ro\/en\/pn-iii-p4-pce-2021-1680","title":{"rendered":"PN-III-P4-PCE-2021-1680"},"content":{"rendered":"
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\n\t\t\t\t\t\tInvestigation of tissular extracellular matrix by automatised 3d tissue reconstruction\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t

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Contract nr. PCE 126\/2022<\/strong><\/span><\/p>

Cod:<\/strong> PN-Ill-P4-PCE.2021-1680<\/span><\/p>

CO:<\/strong> Liehn Elisa<\/span><\/p>

INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI<\/span><\/p>

SI STIINTELOR BIOMEDICALE ”VICTOR BABES”<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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INVESTIGATION OF TISSULAR EXTRACELLULAR MATRIX BY AUTOMATISED 3D TISSUE RECONSTRUCTION<\/span><\/strong><\/p>

\u00a0<\/p>

Despite the huge progress in the last decades, the treatment of cardiovascular diseases lacks in many aspects, since cardiovascular diseases represent are still the main cause of death in develop countries. This is due in part to the controversial data resulting from experimental research, which vary depending on groups and protocols, but also do to lack in methods to analyze such complex data resulting from each experiment.<\/span><\/p>

\u00a0<\/p>

The project offers a solution for standardization and automatisation of analysis of experimental data in fundamental research for cardiovascular diseases. From our knowledge, our employed approach is a novel concept and strategy, representing a radical development of traditional optical imaging processing. By automatically recreating the 3D organ morphology, integrating all the staining and showing the interrelation between all the on-going pathological processes in place, without many human and material resources, we will bring a significant contribution in the cardiovascular research field for future more efficient and reliable animal experiments. Moreover, by development of machine learning-based image analysis system, all information about the quantitative investigation of morphologic and molecular information from previous experiences will be considered. Thus, we align the experimental research with the current regulation of European Union to reduce, refine and replace animal experiments.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project results: (ro vers available) <\/br><\/br>\n\nThe project aimed to standardize tissue imaging and develop and adapt existing software for 3D reconstruction of organs. Different histological and immunofluorescence stains to mark different structures were performed in serial sections, so that the developed software could integrate and overlay all images, allowing a complex 3D reconstruction of pathological processes. Furthermore, it was possible to automatically analyze different structures according to the needs of the different projects. <\/br><\/br>\n\nThe project resulted in: <\/br><\/br>\n\n8 ISI papers, with cumulated IF 39,2\n10 participations to conferences (6 national and 4 international)\n1 patent application<\/strong><\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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PN-III-P4-PCE-2021-1680 Investigarea metricii extracellular tisulare folosind reconstructia 3D automatizata Contract nr. PCE 126\/2022 Cod: PN-Ill-P4-PCE.2021-1680 CO: Liehn Elisa INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE IN DOMENIUL PATOLOGIEI SI STIINTELOR BIOMEDICALE ”VICTOR BABES” INVESTIGAREA MATRICII EXTRACELULARE TISULARE PRIN RECONSTRUC\u021aIE TISULAR\u0102 3D AUTOMATIZAT\u0102 \u00a0 \u00cen ciuda progreselor uria\u0219e din ultimele decenii, tratamentul bolilor cardiovasculare prezint\u0103 lacune \u00een multe aspecte,… <\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-12517","page","type-page","status-publish","hentry"],"yoast_head":"\nPN-III-P4-PCE-2021-1680 - National Institute of Pathology Victor Babe\u015f - Bucharest<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.ivb.ro\/en\/pn-iii-p4-pce-2021-1680\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PN-III-P4-PCE-2021-1680 - National Institute of Pathology Victor Babe\u015f - Bucharest\" \/>\n<meta property=\"og:description\" content=\"PN-III-P4-PCE-2021-1680 Investigarea metricii extracellular tisulare folosind reconstructia 3D automatizata Contract nr. 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