DNA MICROARRAY

                Overview of the equipment that comprises the Agilent DNA SureScan Microarray scanner (purchased through the MODIFICATION 2.2.1 633/2014), Agilent Bioanalyzer, and Equipment support (hybridization oven, vortex, minicentrifugi for the face), computers and specialized programs for political experiments, the extraction and analysis of data).

The equipment for scanning for high-resolution microarray slides, the high sensitivity of the detection system, display, dynamic, allows for the scanning of the blades to the microarray with a high density of oligonucleotide probes

Applications:hybridization, comparative genomic-based microarray array-CGH for the analysis of the imbalances in the genomic and CGH+SNP for the identification of a simultaneous variation in the number of DNA copies, and the regions of the loss of the mutation.

SEQUENCING

Kit for sequencing-Sanger is located in the Laboratory of Medical Genetics, Applied Biosystems 3500 Genetic Analyzer (purchased through the MODIFICATION 2.2.1 633/2014) is an integrated platform that combines hardware performance, the 8-capillary, and programs for direct sequencing, and the analysis of the fragments.

In addition to that, our team used the platform of the sequence is targeted to a new generation of Ion PGM System (ThermoFisher Scientific), located at the Laboratory of Molecular Pathology, Department of Pathology.

Applications:sequencing of target DNA, the analysis of the fragments by capillary electrophoresis, PCR

MOLECULAR GENETICS

Equipment (RCP, Watch the Real-Time PCR Rotor Gene6000, it won't happen, Palm Cycler) and equipment of the general molecular genetics.  

Applications: the technology is based on the PCR

LIGHT MICROSCOPY FOR THE APPLICATIONS OF CYTOGENETICS

Motorized microscope Axio Z1 Zeiss discussed in the light streamed live and epifluorescență, which is equipped with a device-CCD camera-monochrome, high-resolution, the microscope, a Nikon E800 by examining it in the light streamed live and epifluorescență, which is equipped with a device-CCD camera-monochrome, with high-resolution.

The software is Metasystems to scan, detect and capture images automatically to the cytogenetics (the Metafer, and Ikaros), cariotipare (the Ikaros) and the hybrid the in-situ fluorescence (s-M-FISH, m-BAND, Q - FISH & CGH).

Applications: testing of conventional cytogenetics and molecular