PN 23.16.02.03
Approaches to the molecular 3D model (sferoids) edited a gene by the method of CRISPR/Cas9 in the development of solutions for personalized medicine in cancer
The project is funded by the Ministry of Research, Innovation and Digitisation
Project id: PN 23.16.02.03
The duration of the project: 2023-2026
Phone: The National institute of Research and Development in the Field of Pathology, and the Sciences Health, “Victor Babes”
In Charge Of The Project: PI Dr. Codrici Elena
Team: Ana-Maria Enciu, Ionela-Daniela Popescu, Maria Dudau, Lucian Albulescu, Tudor Emanuel Fertig, Aurora Arghir, Sorina Papuc și Cristiana Tanase
Purposedevelopment of experimental models of tumor in 3D in vitro (sferoizi s multicelulari), based on the cells of the edited genes to the genes of an impact on the reprogramming of the metabolic rate (IDH1, CD36), as an innovative approach to screening for resistance to chemotherapy.
The objective of the scientific state:
Objective 1
The generation of experimental models of tumor in 3D in vitro / multicelular sferoids of the cell lines and the standardized (tumor cells, and the cell medium), in order to improve the quality and personalization of the therapy, anti-tumor.
Objective 2
The editing of the dna of the tumor cells of breast cancer (CD36), and glioblastoma (IDH1), through the technology of CRISPR/Cas9 in order to obtain the sferoizi editing gene targeting is the cause of the disease / factors that are affecting the health of; verify and confirm the editing.
Objective 3
The evaluation function of the cells of the edited gene and sferoizilor produced by these cells versus the control (wild type), through its methodology, proteomics, and bioimagistice
Objective 4
The validation of the model sferoizi multicelulari s, editing gene therapy versus control (wild type), in the treatment of these two types of cancer, the identification of new mechanisms of resistance to therapy and the analysis of the differences have arisen as a result of treatment with chemotherapy, between the sferoizii s edit the gene, and the needitați;
The degree of originality and novelty of:
1) the enrichment of the cultures of the tumour in 3D-cell support
2) edit the gene, CRISPR/Cas9
3) the generation of models for the testing of the 3D-edited genes.
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